First drawings of the experimental chamber for my olfactometer

I am delighted to be able to share some of the imagery showing the design of the olfactometer as it currently stands. Hopefully these pictures are worth a thousand words, as today’s blog post is intended to be quite short…

The material being used for the panels and tubing which make the parts illustrated is transparent, colourless, acrylic. The pin which for the hinge and handle for the rotating doors in the mosquito trap/release chambers is stainless steel, as is the mesh which covers the ends and surface of the gate.

3 dimensional drawing of the proposed olfactometer experimental chamber.

3 dimensional drawing of the proposed olfactometer experimental chamber.

With reference to the 3 dimensional diagram above, the air will be blown into the ports at the rear of the unit, where they will pass through the 2 variable chambers. Here the air will flow over the variable/s being tested (or the control), where it will become ‘loaded’ with chemicals which may or may not cause a behavioural change in the mosquitoes. The air will then flow through the main flight chamber before exiting through the mosquito release chamber at the front of the unit.

Plan view of the experimental chamber. 3 dimensional drawings of the proposed olfactometer mosquito release/traps chambers.

Plan view of the experimental chamber. 3 dimensional drawings of the proposed olfactometer mosquito release/traps chambers.

There are a few design points which are currently still under discussion; such as the design of the rotating gates in the mosquito trap/release chambers. Currently they are designed as an acrylic ring which has a stainless steel mesh across it, there is some suggestion that it may be easier to simply use an acrylic disk and drill this through with many small holes. This is the potential drawback that the drilled holes will not provide a visual barrier to the stimulus being offered, where the stainless steel mesh is opaque and will therefore help control against the mosquitoes being able to see rather that smell the stimuli.

Side view of the experimental chamber, showing plane for sectional diagram to the right.

Side view of the experimental chamber, showing plane for sectional diagram to the right.

The airflow inlets shown in sectional view in the 3rd drawing are slightly misrepresented in the drawing. The internal portion, that which is inside the chamber, is actually shorter than shown. This will be adjusted in the next round of design reviews.

As always comments and questions are greatly appreciated!

Meet Ochlerotatus punctor, the puddle dweller!

Today’s blog post is not directly linked to the olfactometer design and build process but instead introduces a little detail around one of the UK’s native mosquitoes.

Both of these are Ochlerotatus punctor and were collected as larvae from the same temporary puddle in the middle of a path through a forest. They were then reared on to adult emergence in the university insectary. It is worth noting that the puddle only lasted about 10 days, yet it yielded many larvae; I do not know however whether the development cycle completed before the puddle dried up. I do know that I did not collect any 4th instars (the final larval stage of mosquitoes) or pupae from the puddle before it dried up.

The images below are included to help highlight some of the physical differences between male and female mosquitoes. The lighting is not great as the photos are taken through the eyepiece of the dissecting microscope in the insectary. These are quite small mosquitoes, about 2/3 the size of the Culiseta species which forms the title image of this blog site.

Ochlerotatus punctor are widely distributed in Britain dependent upon the availability of temporary woodland pools. They females feed on a range of mammals and will readily bite humans, particularly at dusk near aquatic sites (Snow, 1990).

Female Ochlerotatus punctor head with annotations showing pilose antennae and short maxillary palps.

Female Ochlerotatus punctor head with annotations showing pilose antennae and short maxillary palps.

Male Ochlerotatus punctor head with annotations showing plumose antennae and elongate maxillary palps.

Male Ochlerotatus punctor head with annotations showing plumose antennae and elongate maxillary palps.

 

References:

Snow, K.R. (1990). Mosquitoes. Slough: The Richmond Publishing Co. Ltd.

Coming soon – Design Imagery!

One of the failings of this blog is that it is hard for me to describe in words how the olfactometer is going to look, and the principles behind its design. Frustratingly, one of my personal weaknesses is that I am not great at drawing things on a computer!

Fortunately this is where the outreach is paying back! Dave, the aforementioned oil and gas engineer, has offered to work through the design with me in Auto CAD Inventor. Depending upon his availability, and the time that he can afford to donate to the project, I will be able to convey the design far more satisfactorily. We will start with 2 dimensional outlines but potentially can go through to 3d animations even showing the airflow details!

We are going to meet up for the first session this weekend, so I hope to have something to share early next week.

As always please leave a comment or ask any questions that you may have! (Click the “Leave a reply” link below).